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Field tolerance to glufosinate in hybrid corn varieties (non‐GMOs) was evaluated at physiological and biochemical levels. The present experiment was conducted to provide information for the development of a glufosinate‐tolerant corn variety through a conventional breeding program. The effect of glufosinate on the physiological response to crop injury, plant height, and fresh and dry weight was examined in 15 locally adapted corn varieties to this herbicide. The highest degree of tolerance was observed in the varieties Pacific 626 and Pacific 983, while the most susceptible variety was CP 989. CP 989 tended to accumulate ammonia more than Pacific 626 and 983; and the concentration of glufosinate resulting in a 50% increase in ammonia concentration in CP 989 was half that required for Pacific 626 and 983. These results indicate that the degree of ammonia accumulation might be related to the sensitivity of these corn varieties to glufosinate. 相似文献
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MA Hayes BA Quinn ND Keirstead P Katavolos RO Waelchli KJ Betteridge 《Reproduction in domestic animals》2008,43(S2):232-237
A critical period of early gestation in the mare involves the immobilization (fixation) of the encapsulated conceptus at around days 16–17. We compared the major proteins in the normal equine embryonic capsule and endometrial secretions around the period of fixation with those from pregnancies in the process of termination induced by administration of an analogue of prostaglandin F2α (PGF2α ). Uterocalin and β2 -microglobulin (β2 M) associated with the embryonic capsule were proteolytically converted to smaller forms during the fixation period. These conversions were similar in conceptuses from control and treated mares. A 17 kDa cationic protein identified as a secretory phospholipase A2 (sPLA2) type IIA was detected bound to normal capsules but increased substantially in response to PGF2α . Two forms of uteroglobin were distinguished by partial amino acid sequences of ∼6 kDa bands in flush fluids from normal pregnant uteri. After administration of PGF2α one immunoreactive form of uteroglobin was preferentially increased. These studies demonstrate that failure of pregnancy in this model is associated with an increase in secretory phospholipase in the capsule and a change in the forms of uteroglobin in the uterine secretions. 相似文献
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